PROSPERO International prospective register of systematic reviews
The Trp719Arg polymorphism of the KIF6 gene and coronary heart disease risk: systematic review and meta-analysis
Yazmin Hernandez-Diaz, Thelma Beatrizg González-Castro, Carlos Alfonso Tovilla-Zárate, Isela Juárez-Rojop, Manuel Alfonso Baños-González, María Lilia López-Narváez
Yazmin Hernandez-Diaz, Thelma Beatrizg González-Castro, Carlos Alfonso Tovilla-Zárate, Isela Juárez-Rojop, Manuel Alfonso Baños-González, María Lilia López-Narváez. The Trp719Arg polymorphism of the KIF6 gene and coronary heart disease risk: systematic review and meta-analysis.
Available from http://www.crd.york.ac.uk/PROSPERO/display_record.asp?ID=CRD42015024602
In populations with coronary heart disease (CHD), what is the influence of Trp719Arg polymorphism of KIF6 gene in manifesting a cardiovascular disease when compared to a healthy population?
All population based studies reporting the association of Trp719Arg polymorphism of the KIF6 gene and CHD risk were considered. we systematically searched for available articles in multiple electronic databases. The literature search was conducted using PubMed and EBSCO databases. Relevant studies were identified using the terms: “Kinesin 6 AND polymorphisms AND cardiovascular heart disease”, “KIF6 AND polymorphisms AND cardiovascular heart disease”, “KIF6 AND polymorphisms AND CHD”, “KIF6 AND Trp719Arg AND cardiovascular heart disease” and “KIF6 AND Trp719Arg AND CHD”. These words were combined to retrieve the summaries. The search also involved the review of the bibliography cited at the end of the various research articles.
Types of study to be included
In the present report we included cases-control studies which evaluated the association between KIF6 gene with CHD.
Condition or domain being studied
Coronary heart disease. The Trp719Arg polymorphism of the KIF6 gene.
Diagnosis of a cardiovascular disease in the patient study group and controls should be healthy subjects
CAD (Coronary artery disease) was defined as the presence of at least one significant coronary artery stenosis of more than 50% luminal diameter on coronary angiography. Patients with patent coronaries were enrolled as controls.
The control group consisted of subjects without cardiovascular disease.
The following criteria were used to choose suitable studies:
1) to be published in peer-reviewed journals,
2) case-control studies,
3) diagnosis of a cardiovascular disease in the patient study group,
4) the articles had to be written in English.
5) Studies to have the relationship between Trp719Arg with CHD
6) The frequencies of three genotypes should be clearly stated or could be calculated.
The literature was excluded if relevant data were not available and studies in which participants did not have documented symptoms or diagnostic evidence of CHD.
Perform a systematic review and meta-analysis of previously published association studies between the Trp719Arg polymorphism of KIF6 and the development of CHD.
Data extraction, (selection and coding)
The literature search was conducted using PubMed and EBSCO databases. Two researchers (Hernández-Díaz and González-Castro) working independently screened each of the titles, abstracts and full texts to determine inclusion. When the researchers were in disagreement a third researcher (Tovilla-Zárate) was consulted. The same authors mentioned previously, extracted the information from all the included reports. These researchers worked independently and in an agreement with the inclusion criteria listed above. The following data were collected: authors, publication date, study designs, ethnic group, CHD phenotypes, age, gender, characteristics of the controls, total number of cases and controls, and genotypes distributions. If essential information was not presented, authors were contacted for details and the study would be excluded if enough information could not be obtained.
Risk of bias (quality) assessment
Begg’s funnel plot and Egger’s test we will perform to assess the potential publication bias in the available literature. For all these procedures we will use the EPIDAT 3.1 program (http://dxsp.sergas.es).
Strategy for data synthesis
For the meta-analysis the odds ratio (OR) and 95% confidence interval (CI) will be estimated and used to evaluate the strength of the association of KIF6 variant with CHD risk. The pooled ORs will calculated respectively for each of the models used, viz.: allelic (Example: C vs. A), additive (e.g. C/C vs A/A), dominant (e.g. C/C+C/A vs A/A) and recessive (e.g. C/C vs C/A + A/A); OR and 95% CI values will be estimated for each study. For all these procedures we will use the EPIDAT 3.1 program (http://dxsp.sergas.es).Data will analyzed with the random-effects model following the reports in the literature. On the other hand, to explore the problem of publication bias, the Egger´s test and funnel plots will be calculated with the same software.
In order to strengthen the analysis we will evaluate publication bias using the GRADE approach and assessed the risk of bias. The Newcastle-Ottawa Assessment Scale (NOS) will be used for inclusion in the systematic review by scoring the methodological quality.
Analysis of subgroups or subsets
We divided the included studies into CAD and Caucasians populations.
A paper will be submitted to a leading journal in the cardiology area.
Contact details for further information
Dr Hernández Díaz
Andador basquetball, fraccionamiento San Antonio, Cunduacan , Tabasco, Mexico
Organisational affiliation of the review
Dr Yazmin Hernandez-Diaz, Universidad Juárez Autónoma de Tabasco, División Multidisciplinaria de Jalpa de Méndez Dr Thelma Beatrizg González-Castro, Universidad Juárez Autónoma de Tabasco, División Multidisciplinaria de Jalpa de Méndez Dr Carlos Alfonso Tovilla-Zárate, Universidad Juárez Autónoma de Tabasco, División Multidisciplinaria de Jalpa de Méndez Dr Isela Juárez-Rojop, Universidad Juárez Autónoma de Tabasco, División Multidisciplinaria de Jalpa de Méndez Mr Manuel Alfonso Baños-González, Universidad Juárez Autónoma de Tabasco, División Multidisciplinaria de Jalpa de Méndez Miss María Lilia López-Narváez, Universidad Juárez Autónoma de Tabasco, División Multidisciplinaria de Jalpa de Méndez
Anticipated or actual start date
15 June 2015
Anticipated completion date
19 October 2015
Conflicts of interest
Other registration details
Subject index terms status
Subject indexing assigned by CRD
Subject index terms
Coronary Disease; Humans; Polymorphism, Genetic
Any other information
This meta-analysis and updated systematic review of Trp719Arg polymorphism of KIF6 gene will allow better understanding of the role that polymorphism play in the pathogenesis of cardiovascular disease.
Stage of review
Date of registration in PROSPERO
17 July 2015
Date of publication of this revision
17 July 2015
Stage of review at time of this submission
Piloting of the study selection process
Formal screening of search results against eligibility criteria
Risk of bias (quality) assessment
PROSPERO This information has been provided by the named contact for this review. CRD has accepted this information in good faith and registered the review in PROSPERO. CRD bears no responsibility or liability for the content of this registration record, any associated files or external websites.